Cost-effective long-read assembly of a hybrid Formica aquilonia × Formica polyctena wood ant genome from a single haploid individual
| Autor: | Pierre Nouhaud, Jonna Kulmuni, Beresford J |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Contig Hymenoptera 15. Life on land biology.organism_classification 010603 evolutionary biology 01 natural sciences Genome Formica aquilonia 03 medical and health sciences Formica polyctena Evolutionary biology Ploidy Gene 030304 developmental biology Reference genome |
| DOI: | 10.1101/2021.03.09.434597 |
| Popis: | Formica red wood ants are a keystone species of boreal forest ecosystems and an emerging model system in the study of speciation and hybridization. Here we performed a standard DNA extraction from a single, field-collected Formica aquilonia × Formica polyctena haploid male and assembled its genome using ∼60× of PacBio long reads. After polishing and contaminant removal, the final assembly was 272 Mb (4,687 contigs, N50 = 1.16 Mb). Our reference genome contains 98.5% of the core Hymenoptera BUSCOs and was scaffolded using the pseudo-chromosomal assembly of a related species, F. selysi (28 scaffolds, N50 = 8.49 Mb). Around one third of the genome consists of repeats, and 17,426 gene models were annotated using both protein and RNAseq data (97.4% BUSCO completeness). This resource is of comparable quality to the few other single individual insect genomes assembled to date and paves the way to genomic studies of admixture in natural populations and comparative genomic approaches in Formica wood ants. |
| Databáze: | OpenAIRE |
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