SPARC modulates expression of extracellular matrix genes in human trabecular meshwork cells

Autor:	Shaoying Fu, Bingjie Lv, Jing-Lei Liu, Lin Xing, Hai-Ying Wei
Rok vydání:	2011
Předmět:	Small interfering RNA Messenger RNA biology General Medicine Transfection Molecular biology Extracellular matrix Fibronectin Ophthalmology medicine.anatomical_structure RNA interference biology.protein medicine Gene silencing Trabecular meshwork
Zdroj:	Acta Ophthalmologica. 90:e138-e143
ISSN:	1755-375X
DOI:	10.1111/j.1755-3768.2011.02283.x
Popis:	Purpose: To investigate the effects of secreted protein acidic and rich in cysteine (SPARC) on the expression of components of the extracellular matrix (ECM) in cultured human trabecular meshwork (TM) cells. Methods: Cultured human trabecular cells were transfected with small interfering RNAs (siRNAs) specific for the human SPARC gene. Protein and mRNA expressions of fibronectin (FN) and the α1chains of collagen I and collagen III were quantified. Results: After silencing of the SPARC gene by transfection of cells with SPARC siRNA, the expression of COL1A1 and COL3A1 mRNAs and proteins was significantly enhanced, as compared to that in the control group (all, p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8e7235a263239e1f76334e1195b6b856 https://doi.org/10.1111/j.1755-3768.2011.02283.x Zobrazit plný text záznamu Plný text