A flavonoid-rich diet increases nitric oxide production in rat aorta
| Autor: | Juan Sebastián Vera Sánchez, S Benito, P. Puig-Parellada, Diego López, S Buxaderas, Maria Teresa Mitjavila, María P. Sáiz |
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| Rok vydání: | 2002 |
| Předmět: |
Pharmacology
chemistry.chemical_classification medicine.medical_specialty biology Endothelium Superoxide Flavonoid Nitric Oxide Synthase Type III Catechin Nitric oxide Nitric oxide synthase chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Biochemistry Internal medicine biology.protein medicine Quercetin |
| Zdroj: | British Journal of Pharmacology. 135:910-916 |
| ISSN: | 0007-1188 |
| Popis: | 1. Red wine intake is associated with a low risk of cardiovascular disease. This effect has been partly attributed to the action of polyphenolic compounds, which decrease the oxidation of plasma low density lipoproteins. Moreover, nitric oxide ((*)NO) is a vasodilator and polyphenolic compounds induce endothelium-dependent vasorelaxation in vitro. 2. Here we studied whether a diet rich in dealcoholated red wine (DRW) increases acetylcholine-induced vasorelaxation and whether ingestion of DRW-, quercetin- or catechin-rich diets modifies the (*)NO-cyclic guanosine-3',5'-monophosphate (cyclic GMP) pathway and superoxide anion (O2(.-)) release in aorta in a resting state in rats fed semi-purified diets containing either 35% (v w(-1)) DRW, 0.3% (w w(-1)) quercetin or 0.3% (w w(-1)) catechin for 10 days. 3. (*)NO-mediated vasorelaxation induced by acetylcholine was greater in rats fed the DRW-rich diet than in those that received the control diet. 4. Expression of endothelial (*)NO synthase (eNOS) was similar in the four dietary groups. The aortic rings of rats fed either the DRW-, quercetin-, or catechin-rich diets showed higher NOS activity, (*)NO production and cyclic GMP content than those of rats fed the control diet. No changes were observed in O2(.-) production. 5. In summary, diets rich in either DRW, quercetin or catechin induced endothelium-dependent vasorelaxation in rat aorta in a resting state through the enhancement of (*)NO production, without modifying O2(.-) generation, thus the bioavailability of (*)NO was increased. The increase in the (*)NO-cyclic GMP pathway explains the beneficial effect of flavonoids at vascular level. |
| Databáze: | OpenAIRE |
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