Properties of genetically overproducedE. coli xylose isomerase
| Autor: | Michael E. Himmel, Karel Grohmann, S. M. Lastick, M. Y. Tucker, Melvin P. Tucker |
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| Rok vydání: | 1988 |
| Předmět: |
Xylose isomerase
chemistry.chemical_classification Size-exclusion chromatography Bioengineering General Medicine Isomerase Biology Xylose medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology Immunodiffusion chemistry.chemical_compound Enzyme chemistry Biochemistry medicine Polyacrylamide gel electrophoresis Escherichia coli Biotechnology |
| Zdroj: | Biotechnology Letters. 10:79-84 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf01024630 |
| Popis: | Xylose isomerase was purified from a transformedE. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformedE. coli LE392 cells; no differences between these purified enzyme preparations were found. |
| Databáze: | OpenAIRE |
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