Enhanced Bioethanol Production from Blue Agave Bagasse in a Combined Extrusion–Saccharification Process
| Autor: | Eduardo Bárzana, Carmina Montiel, Eduardo Vivaldo-Lima, Martín Hernández-Luna, Oscar Hernández-Meléndez |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
biology Renewable Energy Sustainability and the Environment Chemistry business.industry 020209 energy 02 engineering and technology Cellulase Reactive extrusion 01 natural sciences Biotechnology chemistry.chemical_compound 010608 biotechnology Enzymatic hydrolysis 0202 electrical engineering electronic engineering information engineering biology.protein Xylanase Ethanol fuel Hemicellulose Food science Cellulose Bagasse business Agronomy and Crop Science Energy (miscellaneous) |
| Zdroj: | BioEnergy Research. 9:1005-1014 |
| ISSN: | 1939-1242 1939-1234 |
| DOI: | 10.1007/s12155-016-9747-x |
| Popis: | This paper describes an improved process for bioethanol production using a recently developed combined extrusion–saccharification technology. Blue agave bagasse (BAB) was pretreated via a thermo-mechano-chemical process (co-rotational twin-screw, reactive extrusion) to increase the availability of cellulose and hemicellulose for enzymatic saccharification. Then, several commercial enzyme preparations, boosted with accessory enzymes (exoglucanase, endoglucanase, hemicellulase, xylanase, and β-glucosidase), were tested with extruded BAB at 5 % consistency in a stirred vessel. The enzyme blend that produced the highest saccharification yield was evaluated at different BAB consistencies. The obtained concentration of sugars increased up to 69.5 g/L (73 % yield) when a 20 % BAB mixture was used. When the enzyme blend was fed into the extruder and with a residence time of 2 min, the yield reached 15 % of the maximum theoretical of C6 sugars along this step. This extruded and pre-saccharified BAB was further hydrolyzed and used for fermentation. The pre-saccharification step significantly enhanced cellulose degradation and ethanol production. Our results indicate that the enzymatic saccharification of BAB, coupled with reactive extrusion, produces an excellent substrate for bioethanol production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink