Popis: |
Exposure of P. aurelia to trypsin and chymotrypsin results in the death of a large percentage of the population. Not all antigenic types are equally affected; antigen types 51B and 51E are most affected by exposure to trypsin, while antigen type 51B is most affected by the action of chymotrypsin. Type 51A does not transform when exposed to either trypsin or chymotrypsin, or after the animals have been removed from the enzyme preparations. Type 51B shows little transformations. Types 51C, 51D and 51E transform readily during and after exposure to these two enzymes. In every instance exposure to the enzymes, followed by growth at 20 °, 27 ° or 32 ° results in the transformation to more type 51A than growth at these temperatures alone. Exposure to chymotrypsin leads invariably to more transformation to type 51A than does exposure to trypsin. Exposure of type 51D to either trypsin or chymotrypsin gives rise to type 51J. The obtained results are analogous with those obtained by transformations, resulting from exposure to antiserum or exposure to different temperatures. |