30 Oxidative stress diminishes the number of functional neutrophil granulocytes in lungs of cystic fibrosis (CF) patients, which is ameliorated by glutathione treatment
| Autor: | Peter Oluf Schiøtz, L. Tolbod, K. Bouchelouche, J. Froekiaer, N. Noerskov-Lauritsen, M. Holm |
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| Rok vydání: | 2015 |
| Předmět: |
Pulmonary and Respiratory Medicine
Spirometry medicine.medical_specialty Antioxidant Lung medicine.diagnostic_test business.industry medicine.medical_treatment Inflammation Glutathione medicine.disease medicine.disease_cause Cystic fibrosis Sputum culture chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Internal medicine Pediatrics Perinatology and Child Health Immunology medicine medicine.symptom business Oxidative stress |
| Zdroj: | Journal of Cystic Fibrosis. 14:S64 |
| ISSN: | 1569-1993 |
| DOI: | 10.1016/s1569-1993(15)30207-1 |
| Popis: | Objectives In normal lung tissue PO 2 is highest at the top of the lung. Our aim was to examine if high O 2 tension was reflected in more ROS mediated cell destruction/inflammation and thus a reduction in functionally active neutrophil granulocytes (NG) at the top of the lung compared with the bottom, measured with 18 FDG-PET-CT-scan. We furthermore evaluate the effect of a combined treatment with per oral N-acetylcysteine (100 mg/kg/TID) and inhaled glutathione (600 mg/BID) to reduce a possible state of oxidative stress in CF lungs, measured as an increase in viable NGs. Methods Eight CF children with stable lung condition participated, age 9–14 (5F, 3M), FEV1 (120–78%). All patients were without antiinflammatory medicine 4 weeks before and to the end of the study. Five days before entering all antibiotics and N-acetylcysteine treatment was discontinued. At start patients underwent 18 FDG-PET-CT-scan, L+D, spirometry and sputum culture was taken, and after 14 days all examinations where repeated. Results NG metabolic activity was significantly increased in the lower lobes of the lungs, compared to the top. Comparing left and rigth lungs as separate compartments and including pre- and post-treatment values showed a significant difference (P Conclusion ROS in CF children impairs NG function in a concentration dependent way. Fourteen days with antioxidant treatment increased the number of functionally active NGs significantly. |
| Databáze: | OpenAIRE |
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