The preparation and use of purified and unpurified dispersed adrenal cells and a study of the relationship of their camp and steroid output
| Autor: | R.P. Gould, B.L. Brown, Albano Jd, J.F. Tait, S. A. S. Tait |
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| Rok vydání: | 1974 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class medicine.medical_treatment Biology Biochemistry Angiotensin II Steroid Endocrinology medicine.anatomical_structure Zona fasciculata Zona glomerulosa Internal medicine medicine Corticosteroid Serotonin Steroid 11-beta-hydroxylase Zona reticularis |
| Zdroj: | Journal of Steroid Biochemistry. 5:775-787 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(74)90066-1 |
| Popis: | Zona glomerulosa cells purified from zona fasciculata contamination by unit gravity sedimentation have previously been characterized. Similarly, zona fasciculata cells have now been separated from zona glomerulosa and zona reticularis cells. Purified zona fasciculata cells respond to valine angiotensin II or ACTH. The maximal steroid response is equivalent for both stimuli (about one hundred fold) and also is about the same using purified zona fasciculata cells or unpurified cells from decapsulated tissue. Preparations consisting mainly of zona reticularis cells respond to a lesser extent than pure zona fasciculata cells to both stimuli. The cAMP output for purified glomerulosa cells is increased by high amounts of ACTH, angiotensin II, serotonin and K + (8.4 mM). Submaximal doses of these stimuli, which markedly increase corticosteroid output, do not detectably raise cAMP output. This could be due to kinetic or compartmental factors. However when a maximal steroidogenic dose of cAMP or serotonin (‘pure’ internal cAMP generator) was used to stimulate unpurified or purified glomerulosa cells, steroid output could still be altered by varying K + concentration. This suggests that alteration in cAMP output production is not the only mechanism for controlling steroidogenesis; alteration in cAMP activity by K + or a K + control mechanism independent of cAMP production are possible extra or alternative mechanisms. A formal treatment of the discussion of the role of cAMP in mediating steroidogenesis is presented. |
| Databáze: | OpenAIRE |
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