Biodistribution and tumor uptake of C60(OH) x in mice

Autor: Zheng Wang, Yuangfang Liu, Zhiqiang Ji, Qunying Xie, Haifang Wang, Hongfang Sun
Rok vydání: 2005
Předmět:
Zdroj: Journal of Nanoparticle Research. 8:53-63
ISSN: 1572-896X
1388-0764
DOI: 10.1007/s11051-005-9001-5
Popis: Radiolabeling of fullerol, 125I–C60(OH) x , was performed by the traditional chloramine-T method. The C–I covalent bond in I–C60(OH) x was characterized by X-ray photoelectron spectroscopy (XPS) that was sufficiently stable for in vivo study. Laser light scattering spectroscopy clearly showed that C60(OH) x aggregated to large nanoparticle clumps with a wide range of distribution. The clumps formed were also visualized by transmission electron microscope (TEM). We examined the biodistribution and tumor uptake of C60(OH) x in five mouse bearing tumor models, including mouse H22 hepatocarcinoma, human lung giantcellcarcinoma PD, human colon cancer HCT-8, human gastric cancer MGC803, and human OS732 osteosarcoma. The accumulation ratios of 125I–C60(OH) x in mouse H22 hepatocarcinoma to that in normal muscle tissue (T/N) and blood (T/B) at 1, 6, 24 and 72 h, reveal that 125I–C60(OH) x gradually accumulates in H22 tumor, and retains for a quite long period (e.g., T/N 3.41, T/B 3.94 at 24 h). For the other four tumor models, the T/N ratio at 24 h ranges within 1.21–6.26, while the T/B ratio ranges between 1.23 and 4.73. The accumulation of C60(OH) x in tumor is mostly due to the enhanced permeability and retention effect (EPR) and the phagocytosis of mononuclear phagocytes. Hence, C60(OH) x might serve as a photosensitizer in the photodynamic therapy of some kinds of tumor.
Databáze: OpenAIRE
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