| Autor: |
Charles W Thorpe, George M Ware, Shia S Kuan, Allen S Carman, Jean A Gaul, Octave J Francis |
| Rok vydání: |
1983 |
| Předmět: |
|
| Zdroj: |
Journal of AOAC INTERNATIONAL. 66:587-591 |
| ISSN: |
0004-5756 |
| DOI: |
10.1093/jaoac/66.3.587 |
| Popis: |
A high pressure liquid chromatographic (HPLC) method is described for the determination of xanthomegnin in grains and mixed animal feeds at levels ranging from 150 to 1200 ng/g. This is equivalent to actual amounts of xanthomegnin injected on the HPLC system at from 15 to 120 ng/injection. Xanthomegnin is extracted with chloroform and 0.1M phosphoric acid. An aliquot of the crude extract is purified by column chromatography using a commercially available silica gel cartridge. Xanthomegnin is then separated from the remaining interferences by HPLC with a reverse phase C-8 column, and subsequently determined by absorbance detection at 405 nm. Elapsed time for the method from initial extraction to final HPLC determination is approximately 1 h. Recoveries of xanthomegnin added to grains and animal feeds at levels from 150 to 1200 ng/g averaged 82% with a coefficient of variation of 10.2%. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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