Communication—Microfluidic Electrochemical Acetylcholine Detection in the Presence of Chlorpyrifos
| Autor: | David E. Cliffel, Ethan S. McClain, Dusty R. Miller |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Renewable Energy Sustainability and the Environment Chemistry Microfluidics Condensed Matter Physics Electrochemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Chlorpyrifos Materials Chemistry medicine Acetylcholine medicine.drug |
| Zdroj: | Journal of The Electrochemical Society. 166:G178-G181 |
| ISSN: | 1945-7111 0013-4651 |
| Popis: | An amperometric acetylcholine sensor was developed for use in a microfluidic system and characterized with chlorpyrifos and its metabolite, chlorpyrifos oxon. This enzymatic sensor was highly selective for acetylcholine, with a detection limit of 0.2 μM and sensitivity of 1.7 nA μM−1 from 1–150 μM. Though chlorpyrifos had no effect on sensor function, chlorpyrifos oxon significantly inhibited response across a range of concentrations (0.5–50 μM). Inhibition was reversed by 2-pyridine aldoxime methyl chloride. This platform can be used to both quantify acetylcholine in the presence of chlorpyrifos and as a biorecognition method for chlorpyrifos oxon. |
| Databáze: | OpenAIRE |
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