Interaction of Quillaja bark saponin and bovine serum albumin: Effect on secondary and tertiary structure, gelation and in vitro digestibility of the protein
| Autor: | Joana Léa Meira Silveira, Cíntia Tiemi Misugi Kayukawa, Elaine Kaspchak, Luciana Igarashi-Mafra, Marcos R. Mafra |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Chromatography biology Chemistry Saponin Isothermal titration calorimetry 04 agricultural and veterinary sciences Health benefits biology.organism_classification complex mixtures 040401 food science 01 natural sciences Protein tertiary structure In vitro carbohydrates (lipids) 0404 agricultural biotechnology 010608 biotechnology visual_art Quillaja biology.protein visual_art.visual_art_medium Bark Bovine serum albumin Food Science |
| Zdroj: | LWT. 121:108970 |
| ISSN: | 0023-6438 |
| DOI: | 10.1016/j.lwt.2019.108970 |
| Popis: | The interaction of Quillaja bark saponin and bovine serum albumin was determined by isothermal titration calorimetry and correlated to the structure, gelation and in vitro digestibility of the protein. Results showed that the interactions occur at around 0.5:1.0 mass ratio of saponin to protein at 25 °C resulting in a low heat of interaction and non-specific binding. Formation of saponin and protein complexes was favored at a higher temperature (75 °C), probably due to the exposure of different groups of the protein. The saponin increased the strength of the protein gels, due to the exposure of hydrophobic regions of the protein, as well as due to crosslinking between protein molecules. The protein digestibility was not greatly affected at a saponin to protein mass ratio smaller than 2.5: 1.0. Thus, QBS can be used to increase the protein gel strength and the health benefits of food without a significant impact on protein digestibility. |
| Databáze: | OpenAIRE |
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