Natural Genetic Transformation byAgrobacterium rhizogenes

Autor:	Li-Yan Sun, M. Anis Limami, John P. Helgeson, Corinne Douat, David Tepfer
Rok vydání:	1998
Předmět:	biology Physiology Vegetative reproduction Agrobacterium food and beverages Dwarfism Plant Science biology.organism_classification medicine.disease Transformation (genetics) Anthesis Cichorium Backcrossing Botany Genetics medicine Daucus carota
Zdroj:	Plant Physiology. 118:543-550
ISSN:	1532-2548 0032-0889
Popis:	Genetic transformation of Belgian endive (Cichorium intybus) and carrot (Daucus carota) by Agrobacterium rhizogenes resulted in a transformed phenotype, including annual flowering. Back-crossing of transformed (R1) endive plants produced a line that retained annual flowering in the absence of the other traits associated with A. rhizogenes transformation. Annualism was correlated with the segregation of a truncated transferred DNA (T-DNA) insertion. During vegetative growth, carbohydrate reserves accumulated normally in these annuals, and they were properly mobilized prior to anthesis. The effects of individual root-inducing left-hand T-DNA genes on flowering were tested in carrot, in which rolC(root locus) was the primary promoter of annualism and rolD caused extreme dwarfism. We discuss the possible adaptive significance of this attenuation of the phenotypic effects of root-inducing left-hand T-DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8e0012c10c83e3d4ce365780e2442885 https://doi.org/10.1104/pp.118.2.543 Zobrazit plný text záznamu