Quantification of Platelet-associated IgG for Differential Diagnosis of Patients with Thrombocytopenia

Autor:	H. Kirchner, H Hennig, Harald Klüter, H. Hagenström, P Schlenke
Rok vydání:	2000
Předmět:	medicine.diagnostic_test biology business.industry Autoantibody Hematology Immunofluorescence Immunoglobulin G Flow cytometry Immunopathology Immunology medicine biology.protein Platelet Differential diagnosis Antibody business
Zdroj:	Thrombosis and Haemostasis. 84:779-783
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0037-1614115
Popis:	SummaryImmune thrombocytopenia is due to platelet destruction by circulating glycoprotein-specific antibodies and is found in various disorders. Methods for the detection of platelet-associated IgG (PAIgG) are generally sensitive but unspecific, whereas glycoprotein-specific assays are highly specific but less sensitive. Usefully, a sensitive screening method for PAIgG detection would also provide information for differential diagnosis. We developed a quantitative direct Platelet Immunofluorescence Test (PIFT) by flow cytometry and studied 79 thrombocytopenic patients with immune thrombocytopenia and other disorders. The sensitivity of the assay was 94%, its specificity 66% for the detection of a clinically obvious immune thrombocytopenia. PAIgG levels of patients with immune thrombocytopenia differed significantly from those of other patients with low platelet counts (p
Databáze:	OpenAIRE
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