Functional evaluation of tumor-infiltrating mononuclear cells. Detection of endogenous interferon-γ and tumor necrosis factor-α in human colorectal adenocarcinomas

Autor: Akio Nakane, Misako Asano, Hiroyuki Katoh, Akihiko Numata, Tomonori Minagawa, Tatsuzo Tanabe
Rok vydání: 1991
Předmět:
Zdroj: Cancer. 68:1937-1943
ISSN: 1097-0142
0008-543X
DOI: 10.1002/1097-0142(19911101)68:9<1937::aid-cncr2820680916>3.0.co;2-d
Popis: Quantitative evaluation of the levels of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) in the extracts of tumors and their corresponding normal tissues resected from 43 patients with colorectal adenocarcinoma was done using solid-phase, sandwich radioimmunoassay. The levels of both IFN-gamma and TNF-alpha detected in the tumor tissues were higher than those in the corresponding normal colorectal tissues obtained from each patient. A significant negative correlation was observed between the level of IFN-gamma and TNF-alpha in each tumor extract. The decrease of the level of IFN-gamma in the tumor correlated with the advance of clinical stage, and the levels of IFN-gamma of the patients with distant metastases were significantly lower than those of the patients without distant metastases. However, an increase in the level of TNF-alpha correlated not only with an enlarged diameter but also with the extent of the primary tumor. Immunohistochemical staining of IFN-gamma and TNF-alpha producing cells in tumor tissues showed that IFN-gamma was mainly produced by CD4+ CD8- T-lymphocytes and TNF-alpha was mainly produced by CD11c+ cells with macrophage-like morphology. These results suggest that CD4+ T-lymphocytes that produce IFN-gamma might play an important role in the antitumor response against cancer progression in human colorectal adenocarcinomas.
Databáze: OpenAIRE
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