Synthesis of Fragments of the Vasoactive Intestinal Peptide (VIP) and Analysis of Their Residual Biological Activities

Autor:	Tomislav Barth, Václav Kašička, Jiřina Slaninová, Ivan Svoboda, Pavel Hrbas, Ivana Zoulíková, Jiri Velek
Rok vydání:	1995
Předmět:	chemistry.chemical_classification Capillary electrophoresis Biochemistry In vivo Chemistry Vasoactive Vasoactive intestinal peptide Protein primary structure Peptide Biological activity General Chemistry hormones hormone substitutes and hormone antagonists In vitro
Zdroj:	Collection of Czechoslovak Chemical Communications. 60:1229-1235
ISSN:	1212-6950 0010-0765
DOI:	10.1135/cccc19951229
Popis:	The vasoactive intestinal (poly)peptide (VIP) is a linear peptide containing 28 amino acid residues, whose primary structure indicates a low metabolic stability. The following VIP fragments, as potential metabolites, and their analogues were prepared by synthesis on a solid: [His(Dnp)1]VIP(1-10), VIP(11-14), [D-Arg12]VIP(11-14), [Lys(Pac)15,21,Arg20]VIP(15-22), and VIP(23-28). After purification, the peptides were characterized by amino acid analysis, mass spectrometry, RP HPLC, and capillary zone electrophoresis. In some tests, detailed examination of the biological activity of the substances in vivo and in vitro gave evidence of a low, residual activity of some fragments, viz. a depressoric activity in vivo for [His(Dnp)1]VIP(1-10) and a stimulating activity for the release of α-amylase in vitro and in vivo for [Lys(Pac)15,21,Arg20]VIP(15-22) and VIP(23-28).
Databáze:	OpenAIRE
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