Construction of an Infectious cDNA Clone of the Apple Stem Grooving Capillovirus (Isolate Li-23) Genome Containing a Cauliflower Mosaic Virus 35S RNA Promoter
| Autor: | Hiroshi Magome, Tsuyoshi Takahashi, Nobuyuki Yoshikawa, Hidetaka Terauchi, Narinobu Inouye |
|---|---|
| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Japanese Journal of Phytopathology. 63:432-436 |
| ISSN: | 1882-0484 0031-9473 |
| Popis: | The complete nucleotide sequence of the genome of apple stem grooving capillovirus (ASGV) isolate Li-23 from lily was determined. The isolate Li-23 genome consists of 6496 nucleotides excluding poly(A) tail and contains two overlapping open reading frames (ORFs 1 and 2). ORF1 encodes a protein with a Mr 241, 768 (242K) and ORF2, in a different frame within ORF1, encodes a Mr 36, 179 (36K) protein. The number of nucleotides and the arrangement of ORFs of the isolate Li-23 genome are in perfect agreement with those of isolate P-209 from apple and isolate L from lily. Identities of the nucleotide sequences were 98.4% (Li-23/L) and 83.0% (Li-23/P-209) in whole genomes excluding poly(A) tail. A full-length cDNA clone (pCT35SF) of the isolate Li-23 genome was cloned into a plasmid vector containing cauliflower mosaic virus 35S RNA promoter which was designed to transcribe the isolate Li-23 genome from its first nucleotide. Mechanical inoculation of pCT35SF onto Chenopodium quinoa induced symptoms typical of isolate Li-23 infection. Virus particles, viral RNA and coat protein could be detected in pCT35SF-inoculated plants, demonstrating that pCT35SF is infectious. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|