Enzyme immunoassay with flow-amperometric detection of NADH

Autor:	H M Eggers, H.B. Halsall, William R. Heineman
Rok vydání:	1982
Předmět:	chemistry.chemical_classification Analyte Enzyme Chromatography medicine.diagnostic_test chemistry Immunoassay Biochemistry (medical) Clinical Biochemistry medicine Serum samples Amperometry
Zdroj:	Clinical Chemistry. 28:1848-1851
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/28.9.1848
Popis:	Using phenytoin as a model analyte, we demonstrate that an enzyme-coupled immunoassay based on flow-injection analysis and amperometric detection of NADH is both feasible and practical. Good agreement with a routine clinical laboratory procedure for phenytoin was obtained for patients' serum samples. The electrode must be protected to prevent fouling by proteins in the analytical sample. The optimum detection range for NADH was at 0.01 of the concentrations of NADH generated during the several minutes required for each analysis. This suggests that the electrochemical technique should be extendable to the determination of species at concentrations in the microgram per liter range.
Databáze:	OpenAIRE
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