| Autor: |
Lambertus Dorland, J F Vliegenthart, J. van Pelt, Johannis P. Kamerling, Marinus Duran, C. H. Hokke |
| Rok vydání: |
1990 |
| Předmět: |
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| Zdroj: |
Journal of Biological Chemistry. 265:19685-19689 |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(17)45426-3 |
| Popis: |
Human beta-mannosidosis urine was fractionated by gel permeation chromatography on Bio-Gel P-2 and by high performance liquid chromatography on Partisil 10 SAX. Besides the disaccharide Man beta 1-4GlcNAc as the major component, a sialic acid-containing compound was detected in an amount of 10% compared to that of Man beta 1-4GlcNAc. Structural characterization of the oligosaccharide and of its reduced analogue by sugar composition analysis, methylation analysis, gas-liquid chromatography-mass spectrometry, and 500-MHz 1H NMR spectroscopy gave conclusive evidence for a novel urinary constituent: NeuAc alpha 2-6Man beta 1-4GlcNAc. This linear trisaccharide can be considered as the result of an alpha 2-6-sialylation of the major accumulating compound, Man beta 1-4GlcNAc. The hitherto unknown linkage between sialic acid and mannose was shown to be susceptible to sialidase digestion. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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