GATA-1 Protein Levels Are Not Altered in CD34+ and CD61+ Cells, but NF-κb Protein Levels Are Elevated in Patients with Idiopathic Myelofibrosis
| Autor: | Seema Naik, Tsong H. Chang, Jen-Chin Wang, Chi Chen, Ying-Yi Xiao |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Blood. 112:4498-4498 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v112.11.4498.4498 |
| Popis: | GATA-1 and NF-kB are important in megakaryocytopoiesis and pathogenesis of idiopathic myelofibrosis (IMF). Previous studies have shown that GATA-1 levels were normal in mRNA but were lower in protein content in CD61+ cells of patients with IMF, and we reported previously that GATA-1 protein levels were not altered in CD61+ cells. The current study includes more patients and also measured NF-kB levels. ELISA assay (GATA-1 and NF-kB ELISA kit, Panomics Inc. CA) was used to determine and compare the protein content of nuclear extracts of blood hematopoietic progenitor cells (CD34+) and megakaryocytes (CD61+ cells) from patients with IMF, patients with other myeloproliferative disorders, and with controls. CD61+ cells were obtained from 10-day cultures of blood CD34+ cells in serum-free culture medium with thrombopoietin and stem cell growth factor. Our study confirmed that GATA-1 protein levels are not altered in megakaryocytes (cultured CD 61+ cells) in patients with IMF (Fig. 1) and NF-kB levels are increased (Table 1). We conclude that GATA-1 protein levels are not altered in patients with IMF compared with controls. Fig 1. GATA-1 Protein levels in CD61+ cells Cultured from Patients with IMF & Others Fig 1. GATA-1 Protein levels in CD61+ cells Cultured from Patients with IMF & Others Table 1. NF-kB Levels (ng/2μg protein) IMF Controls CD34+ cells 1.39 ± 0.27 1.10 ± 0.24 CD61+ cells 1.54 ± 0.52 0.45 ± 0.22 |
| Databáze: | OpenAIRE |
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