Application of Fmoc-amino acid carrying an unmasked carbohydrate to the synthesis of the epidermal growth factor-like domain of bovine blood coagulation factor IXElectronic supplementary information (ESI) available: experimental details. See http://www.rsc.org/suppdata/ob/b3/b312413d

Autor:	Takeshi Ishimizu, Sumihiro Hase, Hironobu Hojo, Yoshiaki Nakahara, Toshio Takemura
Rok vydání:	2004
Předmět:	chemistry.chemical_classification Organic Chemistry Peptide Carbohydrate Coagulation Factor IX Biochemistry Amino acid Acylation Protein structure chemistry Epidermal growth factor Physical and Theoretical Chemistry Peptide sequence
Zdroj:	Organic & Biomolecular Chemistry. 2:133
ISSN:	1477-0539 1477-0520
DOI:	10.1039/b312413d
Popis:	The epidermal growth factor (EGF)-like domain of the bovine blood coagulation factor IX (45-87) carrying glucose at Ser(53) was synthesized by a solid-phase method using 9-fluorenylmethoxycarbonyl (Fmoc)-amino acids. The introduction of Ser(53) was carried out using the benzotriazolyl ester of Fmoc-serine carrying an unmasked glucose. The remaining sequence was also introduced using the benzotriazolyl ester. HPLC analysis of the crude peptide shows that acylation of the free hydroxyl group of the glucose was not significant, demonstrating that the amino acid carrying an unmasked carbohydrate is a useful building block for solid-phase synthesis.
Databáze:	OpenAIRE
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