Monte Carlo Poration Model of Cell Membranes for Application to Plasma Gene Transfection
| Autor: | Ahmed Rhallabi, Masafumi Jinno, Mohammed Yousfi, Hideki Motomura, Amel Zerrouki |
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| Rok vydání: | 2015 |
| Předmět: |
animal structures
Polymers and Plastics Chemistry Monte Carlo method Analytical chemistry 02 engineering and technology Plasma Transfection Electron 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 010305 fluids & plasmas law.invention Ion Membrane Magazine law 0103 physical sciences Biophysics 0210 nano-technology Recombination |
| Zdroj: | Plasma Processes and Polymers. 13:633-648 |
| ISSN: | 1612-8850 |
| Popis: | A Monte Carlo poration model is developed to simulate formation pores of few nanometer of width through cell multilayer membranes when impacted by air plasma species. Each species is assumed as a super-particle involving a large particle number. It is assumed a simple membrane model superposing four layers of phospholipids and proteins. Each layer is composed by super-sites subjected to plasma macro-processes (recombination, reflection, activation, opening). Electrons play a main role on super-site activations and openings but ions and radicals have also their own role on pore formation. Judicious choices probabilities data of macro-processes lead to sizes of pores compatible with gene transfection. Present model, validated by comparisons with measured rates of transfected and surviving cells, is a tool for understanding the mechanisms of gene transfection. |
| Databáze: | OpenAIRE |
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