Sampling of the cranium for mitochondrial DNA analysis of human skeletal remains

Autor: Suzanne M. Barritt, Louis N. Finelli, Suni M. Edson, Audrey Meehan, Alexander F. Christensen, Mark Leney
Rok vydání: 2009
Předmět:
Zdroj: Forensic Science International: Genetics Supplement Series. 2:269-270
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2009.09.029
Popis: Sampling of cranial fragments for mitochondrial DNA (mtDNA) analysis is a common practice for identification of skeletonized human remains. The Armed Forces DNA Identification Laboratory (AFDIL) and Joint POW/MIA Accounting Command – Central Identification Laboratory (JPAC-CIL) work in concert to identify the remains of US service members killed in past military conflicts. When dealing with samples taken from remains that are commingled or lack a secure archaeological context, multiple elements must be sampled from the same burial or case. In such cases, the cranium is often fragmentary. Previous work examined the success rate of approximately 4000 skeletal elements for sequencing of mtDNA. The approximately 50% success of the crania seemed anomalous considering the frequency with which they are sampled. Subsequent studies of the 558 cranial fragments tested from 1992 to August 2009 were done to examine the independent rates of success of the portions of the skull. It was found that each yielded reportable mtDNA sequence at rates that exhibited statistically significant differences.
Databáze: OpenAIRE