Influence of different concentrations of ascorbic and gibberellic acids and pH of medium on embryogenesis and regeneration in anther culture of spring triticale
| Autor: | A. K. Daniyarova, A. M. Abekova, G. Kh. Bersimbayeva, K. R. Urazaliyev, R. S. Yerzhebayeva, B. A. Ainebekova, T. A. Bazylova |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant Chemistry medicine.medical_treatment Regeneration (biology) Embryogenesis Stamen Cell Biology Triticale Ascorbic acid 01 natural sciences Agricultural and Biological Sciences (miscellaneous) 03 medical and health sciences Horticulture chemistry.chemical_compound 030104 developmental biology Microspore Genetics medicine Gibberellic acid 010606 plant biology & botany |
| Zdroj: | Cytology and Genetics. 51:448-454 |
| ISSN: | 1934-9440 0095-4527 |
| DOI: | 10.3103/s0095452717060032 |
| Popis: | In this work, the effect of ascorbic and gibberellic acids and pH of medium on embryogenesis and regeneration in anther culture of spring triticale was studied. The study of androgenesis was conducted on two model samples YaTKh-327-11 and YaTKh-18-11. The research revealed that the addition of the antioxidant ascorbic acid at a concentration of 2–8 mg/L into the culture medium to induce embryogenesis enhanced the formation of androgen structures and regeneration of green plants. The addition of gibberellic acid into the culture medium to induce embryogenesis gave negative effects. The average yield of green plants in the anther culture of spring triticale was 2 pcs/100 anthers. According to the research, 277 green plants were received and spontaneous doubling of chromosomes was recorded in 26.7%. |
| Databáze: | OpenAIRE |
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