Evaluation of genetic diversity of honey bee in Ukraine analyzed by the SSR-markers

Autor:	D. I. Hryhorchuk, Ya. V. Pirko, A. M. Rabokon, Ya. B. Blume, N. M. Pirko, A. S. Postovoitova
Rok vydání:	2020
Předmět:	0106 biological sciences 010602 entomology Genetic diversity business.industry fungi behavior and behavior mechanisms food and beverages Honey bee Biology business 01 natural sciences 010606 plant biology & botany Biotechnology
Zdroj:	Faktori eksperimental'noi evolucii organizmiv. 26:56-60
ISSN:	2415-3826 2219-3782
Popis:	Aim. The aim of the work was to analyze current genetic structure of honey bee populations in Ukraine that belong to different subspecies: A. meliffera meliffera, A. meliffera carnica, A. meliffera macedonica using microsatellite markers. Methods. SSR-analysis was used for evaluation of the honey bee polymorphism. Amplified fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The analysis of the sample of honey bees (workers and male-bees) collected from different regions of Ukraine was performed by using two SSR-markers (Ac011 and A007). In this sample reasonably high polymorphism was observed, especially for the SSR-marker A007. Conclusions. It was estimated that SSR-analysis method can be applied in molecular-genetic analysis of honey bees for evaluation of genetic diversity and cross-subspecies hybridization. Keywords: microsatellite markers, Apis meliffera, PIC (Polymorphism Information Content).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8da4479aa96197d691a77c98e05a9ab9 https://doi.org/10.7124/feeo.v26.1241 Zobrazit plný text záznamu