DIVERSITY OF HEPATITIS C VIRUS
| Autor: | Ammara Waqar, Irfan Faroqui, Naeem Shahzad, Sitara Hassan, Hamid Mahmood, Nasir Zulfiqar |
|---|---|
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | The Professional Medical Journal. 21:723-727 |
| ISSN: | 2071-7733 1024-8919 |
| DOI: | 10.29309/tpmj/2014.21.04.2296 |
| Popis: | Objective: (1)To understand the diversity in HCV glycoprotein E1 and E2sequences at different stages of infection. (2) Method widely used in evolutionary studies of HIV,as a new gold standard in HCV research.Setting: Department of Biochemistry Sir Syed Institutest of Medical Sciences Karachi. Period. 1 Jan 2012 to 31 Dec 2012. Design: Experimentalanalysis. Methods: The samples derived from a xenomouse model of transmission and severalsamples from naturally occurring transmission as well as sequences from acute stage ofinfection. we utilized single genome amplification (SGA) technique to recover full- length E1sequences. SGA of full-length E1 glycoprotein sequences, followed by direct sequencingminimizes in vitro generated artifacts and experimental biases associated with the standard bulkamplification and cloning approach, giving an accurate representation of investigated intrahostpopulation. Analysis and results: Comparative analyses of sequences derived from threedifferent settings were performed, in conjunction with a range of phylogenetic tests. As full-lengthE1 sequences were utilised. Conclusions: The role of glycoprotein E1 and E2 during theinfection of HCV was Known. The new gold standard in HCV research prove to be of greatimportance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|