Detection and characterization of autoreactive B cells in patients with Rheumatoid Arthritis (P4024)
| Autor: | Philip Titcombe, Laura Barsness, Lauren Giacobbe, Emily Gillespie, Erik Peterson, Daniel Mueller |
|---|---|
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 190:42.13-42.13 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.190.supp.42.13 |
| Popis: | B cell tolerance to self antigen is critical for preventing several autoimmune diseases, including Rheumatoid Arthritis (RA), but how tolerance is maintained or broken in physiologic settings is not well understood. Our lab recently developed a novel protein-tetramer enrichment strategy for detecting and analyzing rare self antigen-specific B cells within the endogenous polyclonal repertoire of mice. We have now adapted this strategy to study human peripheral blood B cells specific for two different cyclic citrullinated peptides (CCP) that mimic the self-protein epitopes targeted by autoantibodies in a majority of RA patients. RA patients with epitope-specific anti-CCP IgG serum antibodies (Ab+ RA) display an increased frequency of CCP-specific B cells compared to Ab- RA patients and healthy controls. Ab+ RA patients also demonstrate an increased frequency of CCP-specific B cells that have undergone isotype-switching and memory cell differentiation (IgD- CD27+), and their numbers correlate with anti-CCP antibody levels. Future work tracking self antigen-specific B cells in humans will focus on identifying factors important for preserving or restoring B cell tolerance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|