Quantification of cyanazine in water and soil by a magnetic particle-based ELISA

Autor:	David P. Herzog, James R. Fleeker, Fernando M. Rubio, Timothy S. Lawruk, Scott W. Jourdan, Charles E. Lachman
Rok vydání:	1993
Předmět:	Detection limit Chromatography biology Extraction (chemistry) Parts-per notation General Chemistry Standard curve chemistry.chemical_compound chemistry Soil water biology.protein Atrazine Bovine serum albumin General Agricultural and Biological Sciences Triazine
Zdroj:	Journal of Agricultural and Food Chemistry. 41:747-752
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf00029a013
Popis:	A competitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of cyanazine in water and soil was developed using a novel magnetic particle solid phase. Cyanazine was covalently attached to a bovine serum albumin carrier, and the resulting herbicide-protein conjugate was used in rabbits to produce polyclonal antibodies specific for cyanazine. Specificity studies indicate that the antibody can distinguish cyanazine from other structurally similar triazine herbicides, including atrazine. This ELISA has a limit of detection of 0.035 parts per billion (ppb, ng/mL) in water and 3.5 ppb in soil. The standard curve allows quantitation up to 3.0 ppb in water and 300 ppb in soil. The ELISA compares favorably with GC measurements in the analysis of water samples (r = 0.964). Recoveries from fortified soils averaged 86% using a 24-h extraction with methanol/water (75:25 v/v).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8d9283e08a1f7e50fe9bdaeaa835c289 https://doi.org/10.1021/jf00029a013 Zobrazit plný text záznamu