In vivo Effects of DL-α-Difluoromethylornithine on the Polyamine and Nucleotide Phosphate Metabolism in P388/S Leukemia Cells
| Autor: | M. Boldizsár, T. Kremmer, L. Holczinger |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_classification
Spermine Cell Biology General Medicine Metabolism Biology Molecular biology Pathology and Forensic Medicine Ornithine decarboxylase Spermidine chemistry.chemical_compound chemistry Biochemistry Enzyme inhibitor biology.protein Putrescine Nucleotide Polyamine Molecular Biology |
| Zdroj: | Pathobiology. 54:8-15 |
| ISSN: | 1423-0291 1015-2008 |
| DOI: | 10.1159/000163338 |
| Popis: | In vivo effects of DL-α-difluoromethylornithine (DFMO) on the metabolism of polyamines and nucleotide phosphates were monitored in P388/S leukemia cells grown intra-peritoneally in BDF1 inbred male mice. Inhibiting the ornithine decarboxylase (ODC) activity DFMO depleted putrescine and spermidine to 30–50 and 50–60%, respectively, and increased spermine to 25–60% compared with the controls, when given as 2% solution in drinking water of the tumor-bearing animals. DFMO treatment caused a parallel 56% elevation of total nucleotide content in tumor cells with distinct and significant increase of some nucleotide phosphates. The most pronounced alterations were shown in the intracellular UTP (202%), CTP (103%), ADP (92%) and ATP (71%) concentrations. Changes in polyamine and nucleotide phosphate metabolisms were dependent on tumor progression. A possible explanation of the metabolic events induced by DFMO is discussed. |
| Databáze: | OpenAIRE |
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