GC Analysis of Amino Acids Using Trifluoroacetylacetone and Ethyl Chloroformate as Derivatizing Reagents in Skin Samples of Psoriatic and Arsenicosis Patients
| Autor: | Subhan Ali Majidano, Muhammad Yar Khuhawar |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Chromatography Organic Chemistry Clinical Biochemistry Relative standard deviation Biochemistry Analytical Chemistry Amino acid law.invention chemistry.chemical_compound chemistry law Reagent Flame ionization detector Ethyl chloroformate Gas chromatography Derivatization Separation time |
| Zdroj: | Chromatographia. 73:701-708 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/s10337-011-1957-y |
| Popis: | The separation and determination of 19 amino acids were examined using two stages derivatization with trifluoroacetylacetone and ethyl chloroformate from the column HP-5 (30 m × 0.32 mm id) with film thickness 0.25 μm at an initial column temperature 100 °C for 2 min with ramping of 20 °C min−1 up to 250 °C with nitrogen flow rate of 3 mL min−1. The detection was performed by flame ionization detector. Total separation time was 10 min. The separation was repeatable with relative standard deviation (RSD) (n = 5) within 1.5–1.9 and 1.3–1.7% in terms of retention time and peak height/peak area, respectively. The method was applied for the determination of amino acids from skin samples of psoriatic patients (n = 6), arsenicosis patients (n = 5) and normal subjects (n = 9) and variation in the contents of the amino acids was noted. The RSDs for the determination were obtained within 3%. |
| Databáze: | OpenAIRE |
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