Analysis of the Specificity of Auto-Reactive Antibodies to Individual Fragments of the Extracellular Domain of Desmoglein 3 in Patients with Pemphigus Vulgaris
| Autor: | D. A. Dolgikh, E. N. Larina, Dmitry Deryabin, Alexey A. Kubanov, T. V. Bobik, A E Karamova, T. K. Aliev, Mikhail P. Kirpichnikov, A. G. Gabibov, A. A. Nikonorov, Ivan V. Smirnov, M. V. Shpilevaya |
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| Rok vydání: | 2021 |
| Předmět: |
education.field_of_study
medicine.medical_treatment Pemphigus vulgaris Autoantibody General Medicine Biology medicine.disease_cause medicine.disease General Biochemistry Genetics and Molecular Biology Epitope Autoimmunity Antigen Immunology Desmoglein 3 medicine biology.protein Plasmapheresis Antibody education |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 171:475-479 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | A method for the analysis of the epitope specificity of auto-reactive antibodies to desmoglein 3 (Dsg3) using competitive ELISA has been developed. It is based on a two-stage solid-phase ELISA with initial “depletion” of auto-reactive antibodies against the studied epitope and subsequent quantitative assessment of antibodies against full-length extracellular domain Dsg3. The proposed approach for assessing the specificity of the autoimmune response in patients with pemphigus vulgaris can provide in the future the possibility to personalize the therapy using plasmapheresis by preliminary selection of the antigenic composition of the extracorporeal immunosorbent. |
| Databáze: | OpenAIRE |
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