Slit‐scan flow cytometer for recording simultaneous waveforms

Autor:	L. S. Cram, J. D. Parson, M. F. Bartholdi, R. G. Johnston, R. D. Hiebert
Rok vydání:	1985
Předmět:	Materials science business.industry Electromagnetic radiation Slit Light scattering Optics Flow (mathematics) otorhinolaryngologic diseases Waveform Transient (oscillation) business Instrumentation Laser beams Electronic circuit
Zdroj:	Review of Scientific Instruments. 56:691-695
ISSN:	1089-7623 0034-6748
DOI:	10.1063/1.1138207
Popis:	We have developed a slit‐scan flow cytometer for recording the transient, simultaneous light scattering and fluorescence waveforms from individual metaphase chromosomes flowing through a focused laser beam. The two waveforms are recorded on a single‐memory waveform recorder by delaying one of the waveforms and switching between them. The light scattering and fluorescence waveforms contain complementary information on chromosome structure. The delay and switching electronics developed here may be useful in other contexts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8d75dfb4d06d0087ef33ae21b11f018c https://doi.org/10.1063/1.1138207 Zobrazit plný text záznamu