Evidence for the involvement of GTP-binding proteins in the process of anaerobic γ-aminobutyrate accumulation in rice roots

Autor:	Remo Reggiani, Alcide Bertani, Nicoletta Aurisano
Rok vydání:	1996
Předmět:	Ruthenium red GTP' Physiology G protein chemistry.chemical_element Plant Science Trifluoperazine Biology Calcium Transduction (biophysics) chemistry.chemical_compound GTP-binding protein regulators Biochemistry chemistry medicine Agronomy and Crop Science Anaerobic exercise medicine.drug
Zdroj:	Journal of Plant Physiology. 149:517-519
ISSN:	0176-1617
DOI:	10.1016/s0176-1617(96)80327-0
Popis:	Summary The pretreatment of rice roots for 1 h in aerobic conditions with GTPγS or AlF4− induced a higher γ-aminobutyrate (GABA) accumulation during 3 h of anoxia, while GDPβS or GDP slightly inhibited this process. Pretreatment with the Ca2+-channel blocker ruthenium red (RR) or the CaM-antagonist trifluoperazine inhibited the GTPγS- or the AlF4−-stimulated anaerobic GABA accumulation. At elevated RR concentrations, GABA accumulation was completely inhibited both in the absence and presence of AlF4−. Furthermore, western immunodetection with anti-α common antibody revealed a higher amount of Gα-like proteins (30 and 28 kDa) in the presence of GTPγS or AlF4−. These data are discussed in relation to an involvement of G proteins in the transduction af an anaerobic signal.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8d7255498ea4f9bd4f58fc1e199c48de https://doi.org/10.1016/s0176-1617(96)80327-0 Zobrazit plný text záznamu Full Text from ScienceDirect