Phytochemicals, antimicrobial and antioxidant potential of methanolic extract of Berberis aristata roots
| Autor: | Astha Tripathi, Swati Rai, Kunjan Sharma, Sonia Mehta, Monika Thakur, Isha Sharma |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Antioxidant Traditional medicine biology DPPH medicine.medical_treatment Berberis aristata biology.organism_classification Antimicrobial chemistry.chemical_compound food Phytochemical chemistry Herb Berberis medicine Pharmacology (medical) Candida albicans Pharmacology Toxicology and Pharmaceutics (miscellaneous) |
| Zdroj: | RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY. 13:5763-5767 |
| ISSN: | 0974-360X 0974-3618 |
| Popis: | The present study focuses on exploring the presence of phytochemicals, antioxidants and antimicrobial potential of methanolic extract of root of Berberis aristata. The preliminary qualitative analysis of phytochemicals, free radical scavenging activity, antimicrobial potential and FTIR spectrophotometry were investigated from the root extracts of Berberis aristrata. Methanolic extract of root showed the presence of phytochemicals like alkaloids, carbohydrates, flavonoids, phenolic compounds and saponins. Further antioxidant assay like DPPH (Diphenyl -2-Picrylhydrazyl) assay was done. DPPH free radical scavenging activity was expressed in % inhibition. In present study, the % inhibition of methanolic extract was 52% and IC50 value was 26^g/ml. Antimicrobial screening showed good positive results with tested pathogens Staphylococcus aureus, Pseudomonas areginousa, Klebsiella pneumoniae and Candida albicans. The FTIR (Fourier Transform Infrared Spectrophotometer) spectroscopic studies revealed different characteristic peak values which confirmed that there is presence of various phytocompounds such as lipids, proteins in the methanolic root extracts. The FTIR method was performed on a spectrophotometer system, which was used to detect the representative peak values along with their functional groups. The results of the present study generated the FTIR spectrum profile for the medicinally important plant Berberis aristata. This preliminary study shows that root part of Berberis aristata is a promising herb with many phytochemicals, antioxidants and antimicrobial potential. |
| Databáze: | OpenAIRE |
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