Detection of a Tasmanian strain of the biological control agent Enoggera nassaui Girault (Hymenoptera Pteromalidae) using mitochondrial COI

Autor: B. D. Murphy, M. K. Kay, G. R. Allen
Rok vydání: 2004
Předmět:
Zdroj: New Zealand Plant Protection. 57:252-256
ISSN: 1179-352X
1175-9003
DOI: 10.30843/nzpp.2004.57.6902
Popis: Paropsis charybdis (Coleoptera Chrysomelidae) an Australian pest of Eucalyptus in New Zealand is subject to biological control by Enoggera nassaui (Hymenoptera Pteromalidae) a solitary egg parasitoid sourced from Western Australia ( Perth Strain) in 1987 Erratic control in inland regions of New Zealand led to the introduction and release in 2000 of two Tasmanian E nassaui strains to attempt expansion of the climatic range of biocontrol Samples recovered a year later were analysed using partial sequences of the Cytochrome oxidase I (COI) mitochondrial gene to test for establishment This method detected a haplotype corresponding with a Tasmanian (Florentine Valley) strain However as sequences could not be obtained from original Perth strain stocks some doubt remains as to the genetic source of the other current E nassaui populations in New Zealand COI proved adept at distinguishing between different parasitoid populations and shows promise for similar studies
Databáze: OpenAIRE