High temperature induces the synthesis of heat-shock proteins and the elevation of intracellular calcium in the coral Acropora grandis

Autor:	Shiao-ping Huang, Lee-Shing Fang, Ku-lin Lin
Rok vydání:	1997
Předmět:	Molecular mass Coral bleaching Coral chemistry.chemical_element Aquatic Science Calcium Biology medicine.disease_cause Calcium in biology Heme oxygenase chemistry Heat shock protein Botany Biophysics medicine Oxidative stress
Zdroj:	Coral Reefs. 16:127-131
ISSN:	1432-0975 0722-4028
DOI:	10.1007/s003380050066
Popis:	Elevated temperatures induce coral bleaching. To investigate subcellular changes in heat-stressed corals, we examined heat-shock proteins (hsps) and the concentration of calcium in coral cells at elevated temperatures. After heat treatment, three hsps with approximate molecular weights of 70, 60 and 35 kDa were prominent. The 35 kDa heat-shock protein was further demonstrated to be heme oxygenase by immunoblotting, suggesting the involvement of oxidative stress in coral cells during heat treatment. Since the expression of hsps needs the activation of a calcium signal, the change of intracellular calcium concentration in coral cells was examined by a FURA 2 fluorescence method under heat treatment. Intracellular calcium concentration increased at high temperatures with or without the presence of ambient calcium. The extent of the calcium concentration increase was constant for at least 6 hours. This result indicates the existence of an active calcium signal in coral cells at elevated temperatures.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8d46f89c23bce3cff3b04ff2b2473312 https://doi.org/10.1007/s003380050066 Zobrazit plný text záznamu Full text from SpringerLink