Effect of Antidepressants on ATP Content, 3H-Valine Incorporation and Cell Morphometry of Astrocytes Cultured from Rat Brain
| Autor: | Paweł Cieslik, Henryk I. Trzeciak, Anna Pudełko, Andrzej Małecki, Artur Kozłowski, Bożena Gabryel |
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| Rok vydání: | 1995 |
| Předmět: |
Adenosine monophosphate
medicine.medical_specialty Clomipramine Biology Mianserin Imipramine chemistry.chemical_compound Endocrinology medicine.anatomical_structure Developmental Neuroscience Neurology chemistry Internal medicine medicine Antidepressant Maprotiline Adenosine triphosphate medicine.drug Astrocyte |
| Zdroj: | Developmental Neuroscience. 17:292-299 |
| ISSN: | 1421-9859 0378-5866 |
| Popis: | ATP is a potential marker of cell vialibility and growth. The content of ATP and 3H-valine incorporation into proteins were measured and the morphometry was performed after antidepressant treatment of astrocytes cultured in vitro with or without dibutyryl 3′5′-cyclic adenosine monophosphate (dB-cAMP). Antidepressants were added into the culture medium (for 24 h) at a final concentration of 10–4M (imipramine, amitriptyline, clomipramine, dox-epine, mianserin) or 10–5M (maprotiline). It was shown that all antidepressants except maprotiline and imipramine increased ATP level and decreased 3H-valine incorporation into astrocytes. All drugs except clomipramine and maprotiline, diminished cell area and perimeter of astrocytes. The addition of dB-cAMP to cultures caused an increase of astrocyte form factor. It can be concluded that antidepressants have a significant effect on energy metabolism and differentiation of astrocytes cultured in vitro. |
| Databáze: | OpenAIRE |
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