The J Substance of Cattle
| Autor: | J. A. Hayashi, W. H. Stone, K. P. Link, M. R. Irwin |
|---|---|
| Rok vydání: | 1958 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 81:82-90 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Summary Blood group specific substances have been prepared from bovine gastric mucosa. In general, preparations from cows carrying the gene for the J character show high and specific J activity as determined by inhibition tests, while those preparations from cows lacking the gene have no activity. There was no detectable difference in the gross chemical composition of the J active and J inactive preparations. The amounts of the various constituents found (nitrogen content, methylpentose, hexosamine and reducing sugars after hydrolysis) were comparable to those reported by others for the blood group specific substances from various species. The J substance prepared from gastric mucosa and the J substance as it occurs normally on the red blood cells and in the serum behaved similarly in cross-reactions within other blood group systems. Thus, the J active preparations possessed cattle J, sheep R and human A activity, while the J inactive. However, none of the preparations had activity for human blood group B as has been reported by other workers. Further, there was some weak sheep r and strong human O activity among both the J positive and J negative preparations. It has not been possible to produce specific precipitating antisera against the J preparations by immunizing rabbits, cattle or chickens. However, cattle immunized with a J active preparation may have produced precipitins specifically reactive with A active substance from hog, horse and human sources. This paradoxical reactivity for nonhomologous substances has been observed with the naturally occurring anti-J antibodies also. The chicken anti-J sera showed reactions which seemingly were species specific and not J specific. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|