Determination of E vitamers in microalgae using high-performance liquid chromatography with fluorescence detection

Autor: Hans Nelis, AndréP. De Leenheer, Patrick Lavens, Patrick Sorgeloos, Ji-Zeng Huo
Rok vydání: 1997
Předmět:
Zdroj: Journal of Chromatography A. 782:63-68
ISSN: 0021-9673
DOI: 10.1016/s0021-9673(97)00467-6
Popis: No validated high-performance liquid chromatography (HPLC) methods for the determination of vitamin E in algae have been reported. We developed and evaluated a new procedure for the quantitation of microalgal α- and γ-tocopherol (α-T and γ-T) using reversed-phase HPLC with fluorescence detection. The compounds of interest were extracted with a mixture of petroleum ether-diisopropyl ether (3:1, v/v) after saponification in the presence of pyrogallol. δ-Tocopherol (δ-T) or tocol was used as an internal standard. Linearity was achieved (r>0.999) over the range of 0.11∼2.32 μg/,l for α-T and 0.02∼0.23 μg/ml for γ-T. The within-run coefficients of variation for repetitive analysis were 1.9%, 1.7% and 1.0% (n=10) for α-T at concentrations of 0.09, 1.24 and 1.55 μg/ml, and 1.9%, 0.8% and 0.3% (n=10) for γ-T at concentrations of 0.02, 0.08 and 0.23 μg/ml, respectively. The recoveries at low concentration levels (0.11 and 0.02 μg/ml) were 82.4% and 96.2% (n=5) for α-T and γ-T, respectively. For medium concentrations (0.78 and 0.12 μg/ml) and high concentrations (2.32 and 0.20 μg/ml), the recoveries were 94.3∼98.7% for the two tocopherols. The limits of quantitation of α-T and γ-T were 20 and 5 μg/g dry mass, respectively. This method has been routinely used as part of nutritional studies on fish and shrimp larvae fed on microalgae.
Databáze: OpenAIRE
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