| Popis: |
Adipose tissue, an easily available and bioactive tissue, is currently considered as an attractive source of autologous cells for tissue engineering and regenerative medicine. Here we estimated endothelial cell yield of epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT), the two major fat depots, in patients with coronary artery disease. Following enzymatic dissociation of adipose tissue, stromal vascular fraction was cultured in endothelial cell growth medium according to the established protocol. Endothelial phenotype was authenticated by a double immunostaining to VE-cadherin/CD144 and von Willebrand factor. We found that both EAT and SAT contain a comparable and significant amount of endothelial progenitor cells.Adipose tissue, an easily available and bioactive tissue, is currently considered as an attractive source of autologous cells for tissue engineering and regenerative medicine. Here we estimated endothelial cell yield of epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT), the two major fat depots, in patients with coronary artery disease. Following enzymatic dissociation of adipose tissue, stromal vascular fraction was cultured in endothelial cell growth medium according to the established protocol. Endothelial phenotype was authenticated by a double immunostaining to VE-cadherin/CD144 and von Willebrand factor. We found that both EAT and SAT contain a comparable and significant amount of endothelial progenitor cells. |
