Peptide-conjugated liposomes for targeted miR-34a delivery to suppress breast cancer and cancer stem-like population
| Autor: | Zahra Madjd, Seyed Nasser Ostad, Rassoul Dinarvand, Fatemeh Atyabi, Ehsan Khabazian, Faezeh Vakhshiteh |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Liposome
education.field_of_study biology Chemistry CD44 Population Pharmaceutical Science Cancer 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease 030226 pharmacology & pharmacy 03 medical and health sciences 0302 clinical medicine Breast cancer Cancer stem cell Cancer cell biology.protein medicine Cancer research 0210 nano-technology Breast carcinoma education |
| Zdroj: | Journal of Drug Delivery Science and Technology. 57:101687 |
| ISSN: | 1773-2247 |
| Popis: | MicroRNAs perform critical roles in regulation of cancer cells and cancer stem cells (CSCs). CSCs are key contributors of therapy failure; hence, successful tumor therapy requires elimination of both CSCs and tumor cells. MicroRNA-34a (miR-34a) is a well-defined tumor suppressor microRNA which is hypermethylated in breast cancer. Cyclic RGD (cRGD) is a targeting peptide that selectively target integrin receptor-expressing cells. Herein, cRGD-conjugated Polyethylene glycol-modified (PEGylated) liposomes (cRGD-PEG liposomes) encapsulating miR-34a (cRGD-PEG/miR-34a liposomes) was developed for targeted transport of miR-34a into MDA-MB-231 breast cancer cells. A 1.8-fold higher internalization of cRGD-targeted liposomes effectively increased the accumulation of miR-34a in breast carcinoma cells. Notably, cRGD-targeted miR-34a-loaded liposomes showed significant inhibitory outcome on tumor cell growth, migration, and invasion as well as a significant decrease in the percentage of CD44+/CD24−/low cancer stem-like population. The results suggest that cRGD-targeted liposomes may possibly increase the efficiency of therapeutic MicroRNAs in breast carcinoma cells and CSCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect