Polymorphism of four X-chromosomal STR loci in Belarusians and Slovaks

Autor:	Lidia Cybulska, Zuzana Džupinková, A. I. Mikulich, Iosif S. Tsybovsky, Krzysztof Rębała, Daniela Siváková, Joanna Wysocka, Zofia Szczerkowska, Ewa Kapińska
Rok vydání:	2008
Předmět:	Genetics education.field_of_study Pcr cloning Population Biology Molecular biology Pathology and Forensic Medicine Polymorphism (computer science) Multiplex polymerase chain reaction Str loci Statistical analysis Allele education Trinucleotide repeat expansion
Zdroj:	Forensic Science International: Genetics Supplement Series. 1:145-146
ISSN:	1875-1768
Popis:	We have investigated four X-chromosomal STR loci (a tetranucleotide repeat marker HPRTB, DXS7423 and trinucleotide repeat systems DXS101, DXS8377) in population samples of unrelated Belarusian and Slovak males ( N =180 and 116, respectively). Markers were amplified in a multiplex PCR reaction with primers labeled with fluorescent dyes 5-FAM and 5-JOE (dye set F). The separation and detection of PCR products were performed by capillary electrophoresis on a 3130 Genetic Analyzer (Applied Biosystems), using positive controls (K562 and NA9947A) and the GeneScan-500 ROX internal lane standard. Allele designation was based on comparison with the constructed allelic ladder. We performed statistical analysis estimating power of discrimination for males (PD male ). Our data was compared with European populations from Poland and Germany. The obtained data are useful in forensic practice and they contribute to creation of national X-STR databases in Belarus and Slovakia.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8d0bb7c5d422932aa46d0e1ed3214468 https://doi.org/10.1016/j.fsigss.2007.10.142 Zobrazit plný text záznamu Full Text from ScienceDirect