Quantitative Electrophoresis of Serum Proteins on Paper

Autor:	Frederick H. Epstein, Moses Wurm
Rok vydání:	1956
Předmět:	Electrophoresis Chromatography Resolution (mass spectrometry) Logarithm Chemistry Biochemistry (medical) Clinical Biochemistry Analytical chemistry Densitometry Bromphenol Blue Blood proteins Protein concentration Amidoschwarz
Zdroj:	Clinical Chemistry. 2:303-319
ISSN:	1530-8561 0009-9147
Popis:	1. A procedure for paper electrophoresis has been described which gives highly reproducible protein patterns with good resolution and freedom from distortions. 2. Densitometry of protein bands on paper stained with bromphenol blue or Amidoschwarz 10B reveals that the logarithm of protein concentration is proportional to optical density and that Beer's law does not apply. Electrophoretic patterns of normal human serum evaluated in this manner give values in close agreement with those obtained by moving-boundary electrophoresis. 3. Confidence limits were determined for both methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8d0b4ea5e446cfb1e9f623f29b79f62b https://doi.org/10.1093/clinchem/2.5.303 Zobrazit plný text záznamu