Conformational study of poly(dI-dC): Hydrogen–deuterium exchange and low-frequency resonance Raman spectroscopy
| Autor: | D Jancura, Pavol Miskovsky, V. Lisy, G. Fabriciova, Laurent Chinsky |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Aqueous solution Chemistry Organic Chemistry Resonance Raman spectroscopy Analytical chemistry Salt (chemistry) Polymer Spectral line Analytical Chemistry Inorganic Chemistry symbols.namesake Crystallography Polynucleotide symbols Hydrogen–deuterium exchange Raman spectroscopy Spectroscopy |
| Zdroj: | Journal of Molecular Structure. :449-453 |
| ISSN: | 0022-2860 |
| Popis: | Poly(dI-dC) structures in aqueous solutions at different salt conditions were studied by classical Raman spectroscopy monitoring the hydrogen–deuterium (H–D) exchange kinetics in 8-CH group in inosine, and by low-frequency (LF) Raman spectroscopy. It is shown that: (i) the Raman spectrum of the low-salt poly(dI-dC) corresponds to the B conformation of the polynucleotide while the isotopic exchange suggests that this form contains a small portion of the A form, (ii) the high-salt poly(dI-dC) spectrum corresponds to the right-handed polymer with characteristics of both the A and B conformations and the H–D exchange indicates a dominant presence of the A form, and (iii) the LF spectra of the high-salt poly(dI-dC) contain the same features as observed in its low-salt B form and some new peaks which can be attributed to the A form. We conclude that the unusual so-called “X” form of the high-salt poly(dI-dC) is a mixture of the A and B forms with the A form being predominant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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