Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

Autor:	Michael W. Davidson, O. Wolf Lindwasser, Rachid Sougrat, Eric Betzig, Juan S. Bonifacino, George H. Patterson, Jennifer Lippincott-Schwartz, Harald F. Hess, Scott G. Olenych
Rok vydání:	2006
Předmět:	Dronpa Multidisciplinary biology Super-resolution microscopy RESOLFT STED microscopy biology.protein Photoactivated localization microscopy Vinculin Lamellipodium Single Molecule Imaging Cell biology
Zdroj:	Science. 313:1642-1645
ISSN:	1095-9203 0036-8075
Popis:	We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method—termed photoactivated localization microscopy—to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8cea8e2d0710de85bf8cbbd400916195 https://doi.org/10.1126/science.1127344 Zobrazit plný text záznamu Plný text ve formátu PDF