Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription–Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens

Autor: Matthew J. Maenner, Markus H Kainulainen, Wen-Pin Tzeng, Ute Ströher, Jessica A. Belser, Gulchekhra Shakirova, Galina E. Zemtsova, Christopher D. Paddock, Cheng-Feng Chiang, James Graziano, Christopher Sexton, Mike Flint, Bobbie R. Erickson, Brian H. Bird, Kimetha Slater, Brima Kargbo, Janae L. Stovall, Angela J. Sanchez, Amy J. Schuh, Aaron C. Brault, Lisa Wiggleton Guerrero, Robyn A. Stoddard, Ayan K. Chakrabarti, Kimberly A. Dodd, Dianna M. Blau, Alison Jane Basile, Slavica Mijatovic-Rustempasic, Brian R. Amman, Laura K. McMullan, Shelley Brown, Michael K. Lo, Lauren E. Andersen, Tara K. Sealy, Laura Morgan, Shannon L. Emery, Brandy J. Russell, Maryann Turnsek, Molly M. Freeman, Meredith Pyle, Jörn Winter, Marko Zivcec, David Q.-H. Wang, John D. Klena, Jonathan S. Towner, Rachael A. Priestley, Johanna S. Salzer, Raydel Anderson, Samuel J. Crowe, Christin H. Goodman, Scott W. Bearden, Jamie L. Perniciaro, Eric Bergeron, Victor E. Matt-Lebby, Lance Presser, Stuart T. Nichol, Michael D. Bowen, Christina F. Spiropoulou, Feng Liu, Tim Flietstra, Aridth Gibbons
Rok vydání: 2016
Předmět:
Zdroj: Journal of Infectious Diseases. 214:S258-S262
ISSN: 1537-6613
0022-1899
Popis: During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.
Databáze: OpenAIRE