Pachypodostyflavone, a New 3-methoxy Flavone and Other Constituents with Antifilarial Activities from the Stem Bark of Duguetia staudtii
| Autor: | Jean Claude Tchouankeu, Jean-Bosco Jouda, Bruno Ndjakou Lenta, Billy Tchegnitegni Toussie, Alexis Sylvain W. Mbobda, Norbert Sewald, Simeon F. Kouam, Alain Wembe Ngouonpe, Gervais Mouthé Happi, Bel Youssouf G. Mountessou, Elvis Monya |
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| Rok vydání: | 2021 |
| Předmět: |
Stigmasterol
Auranofin biology Traditional medicine 010405 organic chemistry Fractionation biology.organism_classification 01 natural sciences Microfilaria In vitro 0104 chemical sciences Duguetia 010404 medicinal & biomolecular chemistry chemistry.chemical_compound chemistry Annonaceae parasitic diseases medicine Formazan medicine.drug |
| Zdroj: | Planta Medica International Open. 8:e56-e61 |
| ISSN: | 2509-6656 2509-9264 |
| DOI: | 10.1055/a-1492-3585 |
| Popis: | A new flavone derivative named pachypodostyflavone (1), along with 8 known compounds (2–9) and a mixture of β-sitosterol and stigmasterol were isolated from the stem bark of Duguetia staudtii (Annonaceae), based on a bioassay-guided fractionation. Their structures were determined using high-resolution mass spectrometry and NMR spectroscopic data, as well as by comparison with the literature values of their analogs. Selected isolated compounds were evaluated for their in vitro antifilaricidal activities on Onchocerca ochengi microfilariae and adult worms. Inhibition of motility was evaluated spectroscopically on microfilaria and adult male worms. Viability was determined on adult female worms by the MTT/ Formazan assay. Auranofin at 10 µM and 2% DMSO were used as positive and negative controls, respectively. Compounds 1 and 7 showed potent anti-onchocerca activities with 100% activity at 250 µg/mL on both O. ochengi adult male and female worms, while compound 5 displayed 100% activity at 30 µg/mL. |
| Databáze: | OpenAIRE |
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