Ascorbic Acid Content of Blood
| Autor: | Chester J. Farmer, Arthur F. Abt |
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| Rok vydání: | 1935 |
| Předmět: | |
| Zdroj: | Experimental Biology and Medicine. 32:1625-1629 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-32-8205c |
| Popis: | While numerous papers have appeared dealing with the ascorbic acid content of various plant and animal tissues, and urine, only a few refer to the quantity present in the blood. Van Eekelen, Emmerie, Josephy and Wolff,1 and Emmerie and Van Eekelen2 deproteinize blood, blood plasma, or serum with trichloracetic acid, precipitate interfering substances, chiefly SH compounds, with mercuric acetate, then treat with H2S, which not only precipitates the mercury but also reduces that portion of ascorbic acid which in blood occurs in a reversibly oxidized state. The H2S is later removed by a stream of nitrogen, and the ascorbic acid estimated by titration with 2:6 dichlorobenzenoneindophenol.3 Gabbe4 claims that loss of ascorbic acid occurs if a solution of pure ascorbic acid is treated with H2S in the presence of mercuric acetate, and therefore, omits this step. Tauber and Kleiner5 describe a method generally applicable to plant and animal tissues (and to blood) in which the essential features of deproteinizatio... |
| Databáze: | OpenAIRE |
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