Ascorbic acid determination with an automated enzymatic procedure
| Autor: | Susan M. Roberts, Winnie Lee, Robert F. Labbe |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Oxidase test
Chromatography Antioxidant medicine.diagnostic_test Chemistry medicine.medical_treatment Biochemistry (medical) Clinical Biochemistry Ascorbic acid High-performance liquid chromatography Absorbance chemistry.chemical_compound Spectrophotometry medicine Dehydroascorbic acid Quantitative analysis (chemistry) |
| Zdroj: | Clinical Chemistry. 43:154-157 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/43.1.154 |
| Popis: | Given the widespread interest in antioxidant nutrients, we have developed a new procedure that will permit the automated determination of plasma ascorbic acid (AA) concentration with a Roche Fara centrifugal analyzer. After the deproteinization of plasma with metaphosphoric acid, AA is oxidized to dehydroascorbic acid by AA oxidase. The product is coupled to o-phenylenediamine to produce a chromophore for which the absorbance is measured at 340 nm. The procedure allows much faster throughput than conventional HPLC methods while yielding results that correlate well and provide improved precision. Linearity extends beyond the reference range of 26.1–84.6 μmol/L, and severe hemolysis is the only interference identified. |
| Databáze: | OpenAIRE |
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