Preparation and properties of associates of nickel nanoparticles with ss-DNA and proteins
| Autor: | M. A. Zaitseva, I. P. Smirnov, Olga N. Tatarinova, Galina E. Pozmogova, A. N. Chuvilin, V. M. Govorun |
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| Rok vydání: | 2008 |
| Předmět: |
Materials science
Aptamer General Engineering Nanoparticle chemistry.chemical_element engineering.material Condensed Matter Physics Oligomer Combinatorial chemistry law.invention chemistry.chemical_compound Nickel chemistry law engineering Recombinant DNA Organic chemistry Molecule General Materials Science Biopolymer DNA |
| Zdroj: | Nanotechnologies in Russia. 3:391-396 |
| ISSN: | 1995-0799 1995-0780 |
| Popis: | In this paper, we demonstrate that nickel nanoparticles are suitable and promising for forming multifunctional biopolymer structures and have advantages over other nanoplatforms. We found that aggregates of nickel nanoparticles form stable associates with fragments of single-stranded DNA (ss-DNA). The impact of the oligomer length, nucleotide composition, nature of the internucleotide bond, and association conditions on the structure and properties of the complexes was studied. The protein-nickel associates were studied for two recombinant histidine-tagged proteins, for which the isotherms of adsorption were obtained. Biopolymer molecules incorporated in associates do not degrade and can retain (completely or partially) their functionality. Therefore, they can be used for selection of aptamers with binding affinity for proteins. The results contribute to the development of new biopolymer-nickel nanostructures, which have potential as research and diagnostic tools in the fields of molecular nanoelectronics, biology, and medicine. |
| Databáze: | OpenAIRE |
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